COA / HPLC / MS

Calivo Labs — Analytical Documentation

Understanding Our Testing Documentation

Calivo Labs provides three core analytical documents with every production lot: a Certificate of Analysis (COA), an HPLC Analysis Report, and a Mass Spectrometry (MS) Report. Together, these documents answer the three questions that matter most to any serious researcher: Is it pure? Is it what it says it is? And is it safe to work with?

Below is a plain-language explanation of what each document tells you and how to read it.

Certificate of Analysis (COA)

The COA is the master summary document for a specific lot. It lists every test performed, the specification (what the result should be), the actual result, and a pass/fail determination for each test.

Key fields to check on a Calivo Labs COA:

Lot Number — This should match the lot number on your vial label. If it doesn't, contact us.

Product Name and Sequence — Confirms the identity and composition of the blend (BPC-157 + Thymosin Beta-4 Acetate).

Molecular Weight (MS) — The observed molecular weight from Mass Spectrometry testing. This confirms compound identity. BPC-157 should read approximately 1419.55 Da. TB-500 should read approximately 4963.51 Da.

Purity by HPLC — The percentage of the target peptide relative to all detected compounds. Our specification is >98%. Our current lot tested at 99.1%.

Net Peptide Content — The actual mass of active peptide in the vial. This is different from total powder weight (which includes counter-ions and residual moisture). Our current lot showed 11.7mg of net peptide content on a 10mg labeled product.

Endotoxins — The LAL test result. Specification: ≤0.5 EU/mg. "Conforms" means the lot passed.

Microbial Testing — Total Aerobic Count and Yeast & Molds Count. Specification: ≤100 CFU/g. "Conforms" means the lot passed.

Authorization — The COA is signed by our QC Department and dated at time of release. The report status should read "RELEASED — All Tests Passed."

HPLC Analysis Report

HPLC (High-Performance Liquid Chromatography) is the gold standard for measuring peptide purity. The HPLC report contains two critical elements: the chromatogram and the peak table.

The Chromatogram is the visual graph showing detector response (mV) over retention time (minutes). Each peak on the graph represents a detected compound. For a BPC-157 + TB-500 blend, you should see two dominant peaks — one for BPC-157 and one for TB-500 — with a clean, flat baseline between them. A clean baseline means low impurity levels. Noisy baselines or numerous small peaks indicate contamination or degradation.

On the current lot chromatogram, BPC-157 elutes at approximately 9.470 minutes and TB-500 at approximately 11.725 minutes.

The Peak Table lists every detected peak with its retention time, area, height, and area percentage. The two target compound peaks should account for the vast majority of total area. On our current lot, the combined target peak area is 99.1% of the total detected area.

The HPLC report also includes the method details — column type (Phenomenex Luna 3μm C18), mobile phase composition (0.1% TFA in water and acetonitrile), flow rate (0.8 mL/min), detection wavelength (220nm), and gradient program. This information allows any qualified laboratory to reproduce the test independently.

Mass Spectrometry (MS) Report

Mass Spectrometry confirms compound identity by measuring exact molecular weight. While HPLC tells you how pure something is, MS tells you what it actually is.

The MS report shows a spectrum with detected ion peaks. Each major ion corresponds to a charge state of the molecule. The report includes key ion assignments showing the relationship between observed mass-to-charge (m/z) ratios and the intact molecular weight.

For BPC-157, the expected molecular weight is 1419.55 Da. For TB-500 (Thymosin Beta-4), the expected molecular weight is 4963.51 Da. If the observed values fall within ±1.0 Da of the theoretical weight, the identity is confirmed. On our current lot, BPC-157 was observed at 1418.70 Da and TB-500 at 4963.60 Da — both within specification.

This is the test that proves you are not receiving a cheaper substitute peptide, a degraded compound, or mislabeled material.

How to Access Your Documentation

All three documents — COA, HPLC Report, and MS Report — are embedded directly on each product page under the COA / HPLC / MS tab. They are also available for download. Documentation is updated whenever a new production lot is released. If your vial lot number does not match the documentation currently displayed, contact us at [support@calivolabs.com] and we will provide the batch-specific reports for your lot.

A Note on Documentation Integrity

Every document published on calivolabs.com is an authentic representation of analytical testing conducted on the stated lot number under GLP-compliant conditions. Additionally, our independent third-party verification by Frontage Laboratories (Exton, PA) provides a separate layer of confirmation beyond our in-house documentation. The Frontage test report is available on every product page under the 3rd Party Testing tab.

We do not recycle reports from previous batches. We do not use generic certificates. We do not alter analytical data. What the lab measured is what you see.